Identification of a 'snapshot' of co-expressed angiogenic markers in laser-dissected vessels from unstable carotid plaques with targeted arrays.

2.50
Hdl Handle:
http://hdl.handle.net/2173/109600
Title:
Identification of a 'snapshot' of co-expressed angiogenic markers in laser-dissected vessels from unstable carotid plaques with targeted arrays.
Authors:
Slevin, Mark; Turu, Marta M.; Rovira, Norma; Luque, Ana; Baldellou, M.; Krupinski, Jurek; Badimon, Lina
Citation:
Journal of vascular research, 2010, vol. 47, no. 4, pp. 323-35
Publisher:
S. Karger AG
Issue Date:
2010
URI:
http://hdl.handle.net/2173/109600
DOI:
10.1159/000265566
PubMed ID:
20016206
Additional Links:
http://www.karger.com/JVR
Abstract:
BACKGROUND/AIMS: Angiogenesis is a feature of the atherogenic process, with intimal neovascularisation arising from vessels in the adventitia, adjacent to a plaque. Immature, leaky blood vessels from unstable plaques proliferate abnormally and, being poorly invested with smooth muscle cells, may contribute to instability of the plaque by facilitation of inflammatory cell infiltration and haemorrhagic complications. METHODS: We used laser-capture microdissection to isolate angiogenic areas of the extracellular matrix (containing CD105/flt-1-positive, fragile thin-walled vessels) and non-angiogenic vascular areas (CD105-negative, with smooth muscle cell covering) of complicated endarterectomy plaques, and specifically designed angiogenesis-TaqMan real-time PCR microarrays to identify gene expression. RESULTS: Important pro-angiogenic components, including Notch-3, delta-like-4 (DLL4), Tie-2, angiopoietin-1 (Angio-1) and receptor for advanced glycation end products (RAGE), and one anti-angiogenic factor, endostatin, were up-regulated in these regions. Immunohistochemistry demonstrated localisation within intimal, active (CD105-positive) microvessels and co-localisation of Notch-3 and DLL4/Tie-2 and Angio-1 in the same vessels indicating multiple/synergistic signalling mechanisms associated with vessel development. CONCLUSION: These data, although providing only a snapshot of information, demonstrate that plaque vascularisation occurs in the presence of multiple angiogenically active factors. Knowledge of their combined effects could help in the formulation of novel therapeutics designed to stabilise or prevent their formation in the treatment of atherosclerosis.
Type:
Article
Language:
en
Description:
Full-text of this article is not available in this e-prints service. This article was originally published following peer-review in Journal of Vascular Research, published by and copyright S. Karger AG.
Keywords:
Angiogenesis; Atherosclerosis; Carotid plaques; Laser-capture microdissection
MeSH:
Aged; Angiogenic Proteins; Angiopoietin-1; Antigens, CD; Carotid Arteries; Carotid Stenosis; Dissection; Endarterectomy, Carotid; Endostatins; Gene Expression Profiling; Genetic Markers; Humans; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; Lasers; Male; Middle Aged; Neovascularization, Physiologic; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Receptor, TIE-2; Receptors, Cell Surface; Receptors, Immunologic; Receptors, Notch; Rupture; Vascular Endothelial Growth Factor Receptor-1
ISSN:
1423-0135; 1018-1172

Full metadata record

DC FieldValue Language
dc.contributor.authorSlevin, Marken
dc.contributor.authorTuru, Marta M.en
dc.contributor.authorRovira, Normaen
dc.contributor.authorLuque, Anaen
dc.contributor.authorBaldellou, M.en
dc.contributor.authorKrupinski, Jureken
dc.contributor.authorBadimon, Linaen
dc.date.accessioned2010-08-13T13:30:56Z-
dc.date.available2010-08-13T13:30:56Z-
dc.date.issued2010-
dc.identifier.citationJournal of vascular research, 2010, vol. 47, no. 4, pp. 323-35en
dc.identifier.issn1423-0135-
dc.identifier.issn1018-1172-
dc.identifier.pmid20016206-
dc.identifier.doi10.1159/000265566-
dc.identifier.urihttp://hdl.handle.net/2173/109600-
dc.descriptionFull-text of this article is not available in this e-prints service. This article was originally published following peer-review in Journal of Vascular Research, published by and copyright S. Karger AG.en
dc.description.abstractBACKGROUND/AIMS: Angiogenesis is a feature of the atherogenic process, with intimal neovascularisation arising from vessels in the adventitia, adjacent to a plaque. Immature, leaky blood vessels from unstable plaques proliferate abnormally and, being poorly invested with smooth muscle cells, may contribute to instability of the plaque by facilitation of inflammatory cell infiltration and haemorrhagic complications. METHODS: We used laser-capture microdissection to isolate angiogenic areas of the extracellular matrix (containing CD105/flt-1-positive, fragile thin-walled vessels) and non-angiogenic vascular areas (CD105-negative, with smooth muscle cell covering) of complicated endarterectomy plaques, and specifically designed angiogenesis-TaqMan real-time PCR microarrays to identify gene expression. RESULTS: Important pro-angiogenic components, including Notch-3, delta-like-4 (DLL4), Tie-2, angiopoietin-1 (Angio-1) and receptor for advanced glycation end products (RAGE), and one anti-angiogenic factor, endostatin, were up-regulated in these regions. Immunohistochemistry demonstrated localisation within intimal, active (CD105-positive) microvessels and co-localisation of Notch-3 and DLL4/Tie-2 and Angio-1 in the same vessels indicating multiple/synergistic signalling mechanisms associated with vessel development. CONCLUSION: These data, although providing only a snapshot of information, demonstrate that plaque vascularisation occurs in the presence of multiple angiogenically active factors. Knowledge of their combined effects could help in the formulation of novel therapeutics designed to stabilise or prevent their formation in the treatment of atherosclerosis.en
dc.language.isoenen
dc.publisherS. Karger AGen
dc.relation.urlhttp://www.karger.com/JVRen
dc.subjectAngiogenesisen
dc.subjectAtherosclerosisen
dc.subjectCarotid plaquesen
dc.subjectLaser-capture microdissectionen
dc.subject.meshAged-
dc.subject.meshAngiogenic Proteins-
dc.subject.meshAngiopoietin-1-
dc.subject.meshAntigens, CD-
dc.subject.meshCarotid Arteries-
dc.subject.meshCarotid Stenosis-
dc.subject.meshDissection-
dc.subject.meshEndarterectomy, Carotid-
dc.subject.meshEndostatins-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGenetic Markers-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIntercellular Signaling Peptides and Proteins-
dc.subject.meshLasers-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeovascularization, Physiologic-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshReceptor, TIE-2-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshReceptors, Immunologic-
dc.subject.meshReceptors, Notch-
dc.subject.meshRupture-
dc.subject.meshVascular Endothelial Growth Factor Receptor-1-
dc.titleIdentification of a 'snapshot' of co-expressed angiogenic markers in laser-dissected vessels from unstable carotid plaques with targeted arrays.en
dc.typeArticleen

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